Immobilization of Pseudomonas fluorescens Lipase on Hollow Poly(o-phenylenediamine) Microspheres and Its Application in the Preparation of Citronellyl Acetate

In order to address the challenges associated with free lipase in organic solvents, including aggregation, poor stability, and low catalytic efficiency, this study developed two types of poly(o-phenylenediamine) microspheres (solid hollow) as supports for immobilizing lipase. The immobilization process utilized an adsorption method, hollow being identified optimal support a 2:5 enzyme-to-support ratio. On basis, was immobilized by covalent binding method. conditions consisted treating 2% glutaraldehyde at 40 °C 2 h pH 7.0 buffer. specific activity enzyme 5.3 times higher than that enzyme. Covalent-binding also used preparation citronellyl acetate transesterification reaction, and, optimized reaction where amount 0.1 g/mL, temperature 50 shaking speed 200 r/min during reaction. Under these conditions, yields can exceed 99% after h. Furthermore, stability investigated, residual 95% seven repetitions, while only 70%. After 56 days storage room temperature, retained 60% its original viability, 31%.